摘要:
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目的建立鸡骨草药材的指纹图谱。方法采用RP鄄HPLC 高效液相色谱法,Phenomenex鄄ODS 色谱柱(250 mm 伊4. 0 mm,5 滋m),甲醇-0. 5%冰醋酸溶液,梯度洗脱,流速1. 0 ml/ min,检测波长270 nm。结果建立了鸡骨草药材指纹图谱共有模式,显示5 个共有峰,指认了相思子碱1 个特征峰。结论HPLC 指纹图谱中各峰分离度较好,方法具有较好的精密度、重现性和稳定性,符合指纹图谱的要求,可用于鸡骨草药材质量控制。 关 |
Objective To establish a HPLC fingerprint analysis method for Abrus cantoniensis. Methods Phenomenex-ODS column (250 mm 4. 0 mm, 5 m) was used, with gradient elution by the mobile phase consisted of Methanol-0. 5% glacial acetic acid. The flow rate was 1. 0 ml/ min, and the detection wavelength was 270 nm. Results A mutual model of finger-print chromatogram was established. 5 common peaks were displayed. A characteristic peak of abrine was identified. Conclusion The characteristic peaks are selected with good resolution, and the method is reproducible, accurate, and stable. It can be used for the quality control of Abrus cantoniensis. |
基金项目:
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作者简介:
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黄勇斌,副主任中药师,Tel:13005763641,E-mail:hyb1993@21cn. com |
通讯作者: |
参考文献:
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[1] 国家药典委员会. 中国药典(一部) [S]. 北京:中国医药科技出版社,2010:180. [2] 史海明,温晶,屠鹏飞. 鸡骨草的化学成分研究[J].中草药,2006, 37 (11) : 1610 -1613. [3] 史海明,温晶,屠鹏飞. 毛鸡骨草的化学成分研究[J]. 中草药,2006, 37 (5) : 658 -660. [4] 史海明,黄志勤,温晶,等. HPLC 法测定鸡骨草药材中相思子碱的含量[J]. 药物分析杂志,2007,27(11):1716 -1718. [5] 肖崇厚. 中药化学[M]. 上海:上海科学技术出版社,1996:118. |
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