设为首页 |  加入收藏
首页期刊介绍编委会投稿须知通知公告刊登文章继续教育园地协办单位联系我们广告合作
公告栏:
超临界CO2 抗溶剂法制备左旋聚乳酸微粒的工艺研究
作者:颜庭轩 王志祥 黄德春   孙思杭 党蓓蕾 
单位:中国药科大学制药工程教研室,江苏南京210009
关键词:超临界抗溶剂法 PLLA 玻璃化温度 药物载体 过饱和度 
中图分类号:R943
文献标志码:A
出版年,卷(期):页码:2013,23(1):4-7,
收稿日期:2012 -10 -15
摘要:
目的采用超临界抗溶剂法(SAS)制备可生物降解的左旋聚乳酸(PLLA)超细微粒。方法以二氯甲烷和丙酮为溶剂,CO2 为抗溶剂,制备得到PLLA 微粒,研究各种操作参数对产品质量的影响。结果成功制备得到粒径小(约8 μm)、分布较窄的PLLA 微粒。单因素实验表明:操作压力越高粒径越小;温度大于50 ℃时微粒易团聚;溶液浓度越高,微粒粒径越大;选用一定比例的二氯甲烷-丙酮混合溶剂有助于得到粒径小、分布均匀的微粒。结论正交试验得到优选工艺为:浓度8 mg/ mL,结晶压力16 MPa,温度39 ℃,二氯甲烷:丙酮=2∶1。
Objective To prepared Poly L-lactide acid ( PLLA) by using supercritical CO2 anti-solvent ( SAS) technology. Methods PLLA were prepared by SAS with dichloromethane and acetone as the cosolvent and CO2 as the anti-solvent. The influence of operating parameters on the product quality was studied. Results Microparticle of PLLA were successfully prepared with a narrow particle size(8 μm) approximately. The size of PLLA microparticle decreased along with the operating pressure increasing. There would be agglomerated particles when the operating temperature was above 50 ℃. The particle size increased when the solution concentration increased. The mixture of methylene chloride and acetone mixed solvent would be helpful to obtain narrow particle size distribution for PLLA. Conclusion The orthogonal experiment optimization process conditions are set as follows: PLLA concentrationis 8 mg/ mL, crystallization pressureis 16 MPa, crystallization temperature is 39 ℃, and cosolvent ratio dichloromethane:acetone =2∶1.
基金项目:
江苏省自然科学基金资助项目(编号:BK2012763) 大学生创新药物研制能力提高项目(国家级大学生创新创业训练计划项目)资助项目(编号:J1030830)
作者简介:
颜庭轩,在读研究生,从事制药分离方向的研究
通讯作者:王志祥,教授,博士生导师,从事制药工程领域的教学与科研,Tel:025-83271258,E-mail chinawzx@ sohu. com
参考文献:
[1] 蒲曦鸣,康云清,陈爱政,等. 超临界CO2 抗溶剂法制备聚乳酸药物缓释微球[J]. 功能材料,2007,38 (4):549 -555.
[2] Biggs DL,Lengsfeld CS,Hybertson BM,et al. in vitro and vivo evaluation of the effects of PLA microparticle crystallinity on cellular response [J]. Journal of Controlled Release,2003,92(1):147 -161.
[3] 黄德春,刘巍,乐龙,等. 超临界流体抗溶剂结晶流程设计与装置研制[J]. 南京理工大学学报(自然科学版),2010,34(1):146 -150.
[4] 蔡锦源,黄德春,王志祥,等. 超临界抗溶剂技术在药物微粒制备领域中的应用[J]. 今日药学,2011,21(12) :768 -772.
[5] 巫先坤,黄德春,王志祥,等. 超临界抗溶剂装置的现状及其展望[J]. 化工进展,2012,31(3):489 -494.
[6] Lee CW,Kima SJ,Youn YS,et al. Preparation of bitter taste masked cetirizine dihydrochloride/ β-cyclodextrin inclusion complex by supercritical anti-solvent ( SAS)process[J]. The Journal of Supercritical Fluids,2010,55(1):348 -357.
[7] 蒲曦鸣,康云清,陈爱政,等. 超临界CO2 抗溶剂法制备聚乳酸药物缓释微球[J]. 功能材料,2007,4(38):549 -552.
[8] 滕新荣,任杰,张鹏. 超临界抗溶剂技术制备聚乳酸微粒及表征[J]. 高分子材料科学与工程,2006,1(22):195 -198.
[9] Kwak H,Bae SY. Recrystallization of L-PLA fine Particles by supercritical Anti-solvent (SAS) Process [J].Journal of industrial and engineering chemistry,2006,12(3):387 -394.
服务与反馈:
文章下载】【发表评论】【查看评论】【加入收藏
提示:您还未登录,请登录!点此登录
 
友情链接  
广东今日药学杂志社有限公司   地址:广州市东风东路753号东塔7楼702
电话:020-37886325       邮箱:jinriyaoxue@163.com
本系统由北京博思汇文数字科技有限公司设计开发 技术服务电话:400-921-9838
粤ICP备20054928号

粤公网安备 44010402002138号

您是第 165215 位访问者