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水飞蓟煎剂治疗急性鹅膏肽类毒素致肝损伤的实验研究
作者:张紫萍1 凌汉新1  青1 武大林2 
单位:1. 广州市第十二人民医院,广东 广州 510620 ;2. 南方医科大学南方医院中心实验室,广东 广州 510515
关键词:水飞蓟 灰花纹鹅膏菌 SD 大鼠 肝功能 RNA 聚合酶 
中图分类号:R282. 7
文献标志码:A
出版年,卷(期):页码:2014,24(1):11-14,
收稿日期:2013 - 09 - 17
摘要:
目的 探讨水飞蓟煎剂治疗急性鹅膏肽类毒素所致肝损伤的解毒机制。 方法 选用健康 SD 大鼠 32 只,随机分成 4 组(每组 8只):正常对照组、中毒对照组、水飞蓟煎剂1 g / mL 组、水飞蓟煎剂3 g / mL 组。 除正常对照组外,其余 3 组均制成急性鹅膏毒蕈中毒鼠模型。 造模后每日给予相应药物,3 d 后观察大鼠的存活情况,并采血检查谷丙转氨酶(ALT)、谷草转氨酶(AST)和用紫外分光光度法测定肝细胞 RNA 聚合酶活性。 结果 中毒对照组的血浆 ALT 和 AST 较正常对照组明显增加(P < 0. 01),不同剂量水飞蓟煎剂灌胃治疗3 d 后,2 组血浆ALT 和AST 较中毒对照组明显降低,2 组间比较差异有统计学意义;中毒对照组肝细胞RNA 聚合酶活性明显低于正常对照组(P < 0. 01), 水飞蓟煎剂治疗组肝细胞 RNA 聚合酶活性明显高于正常对照组(P < 0. 01), 同时治疗组之间呈量 -效关系(P < 0. 01)。 结论 水飞蓟煎剂对鹅膏毒蕈中毒肝有明显保护作用, 且作用呈量 -效关系,其机制可能与提高肝细胞 RNA 聚合酶活性有关。
基金项目:
广东省科技计划项目(编号:2009B060700021)
作者简介:
张紫萍,副主任药师,Tel:13650801165,E-mail: zzp08@126. com
通讯作者:
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