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SPG膜乳化法制备载蛋白的PEG-PLGA复合微球
作者:郭喆霏1  钟晨2  罗永梅2  罗宇燕1  张永明1 
单位:1. 中山大学附属第三医院药剂科, 广东 广州 510630;
2. 中山大学药学院, 广东 广州 510006
关键词:缓释微球 聚乙二醇-聚乳酸聚乙醇酸 蛋白药物 海藻酸钠 SPG膜乳化法 
中图分类号:R460.6
文献标志码:
出版年,卷(期):页码:2017,27(1):20-23,
收稿日期:2016-11-18
摘要:
目的 考察在内水相加入海藻酸钠(Sa),外水相加入氯化钙(CaCl2),对SPG膜乳化法制备聚乙二醇-聚乳酸聚乙醇酸(PEG-PLGA)微球的载药释药特性的影响。方法 扫描电镜观察微球内外形态,并计算其结构参数;激光共聚焦显微镜观察药物在微球内部的分布情况;并考察微球的包封率及体外释药行为。结果 加入海藻酸钠和氯化钙制得的复合微球孔隙率低,蛋白分布广,并具有较高的包封率,前期释放低,释放无迟滞。结论 PEG-PLGA复合微球载药释药性能较好,释药符合缓释制剂标准。
OBJECTIVE To investigate the influences of adding sodium alginate to the inner aqueous phase and CaCl2 to the outer aqueous phase on the physical and drug release properties of protein loaded PEG-PLGA microspheres prepared by SPG membrane emulsification. METHODS Surface and sectional morphology were analyzed by scanning electron microscopy and drug distribution was studied by confocal laser scanning microscopy. The encapsulated efficiency and release properties were also accessed. RESULTS Addition of alginate and CaCl2 resulted in higher EE, lower porosity, and increased in homogeneity of drug distribution. Moreover, it showed a suitable protein release profile without lag phase. CONCLUSION The result shows that PEG-PLGA composite microspheres are of better drug-loading and release properties.
基金项目:
广东省医院药学科研基金(2015SW12)
作者简介:
郭喆霏,药师,研究方向:缓释制剂研究,Tel:020-85252435,E-mail:59028504@qq.com
通讯作者:罗宇燕,主管药师,Tel:020-85252100,E-mail:27132889@qq.com
参考文献:
[1] Kazazi-Hyseni L, Landin M, Lathuile A, et al. Computer modeling assisted design of monodisperse PLGA microspheres with controlled porosity affords zero order release of an encapsulated macromolecule for 3 months[J]. Pharmaceutical Research, 2014, 31(10):2844-2856.
[2] Pai SS, Tilton RD, Przybycien TM. Poly(ethylene glycol)-modified proteins:implications for poly(lactide-co-glycolide)-based microsphere delivery[J]. AAPS Journal, 2009,11(1):88-98.
[3] Giteau A, Venier-Julienne MC, Aubert-Pouessel A, et al. How to achieve sustained and complete protein release from PLGA-based microparticles[J]. International Journal of Pharmaceutics, 2008, 350(1-2):14-26.
[4] Patel B, Gupta V, Ahsan F. PEG-PLGA based large porous particles for pulmonary delivery of a highly soluble drug, low molecular weight heparin[J]. Journal of Controlled Release, 2012, 162:310-320.
[5] 罗宇燕, 张永明, 吴传斌. 一种聚乳酸聚乙醇酸缓释微球的制备工艺[J].中国医院药学杂志, 2011, 31(11):875-878.
[6] 黎呐, 麦海燕, 罗宇燕, 等. 聚乳酸聚乙醇酸微球冷冻切片方法的研究[J]. 广东药学院学报, 2014,30(1):1-5.
[7] 钟晨, 罗宇燕, 郭喆霏, 等. SPG膜乳化法制备PEG-PLGA微球和PLGA微球载药释药特性的对比研究[J]. 广东药学院学报, 2016, 32(3):268-274.
[8] Mao S, Xu J, Cai C. Effect of WOW process parameters on morphology and burst release of FITC-dextran loaded PLGA microspheres[J]. International Journal of Pharmaceutics, 2007, 334(1-2):137-148.
[9] Dash S, Murthy P, Nath L, et al. Kinetic modeling on drug release from controlled Drug delivery systems[J]. Acta Poloniae Pharmaceutica-Drug Research, 2010, 67(3):217-223.
[10] Gainza G, Aguirre JJ, Pedraz JL, et al. rhEGF-loaded PLGA-Alginate microspheres enhance the healing of full-thickness excisional wounds in diabetised Wistar rats[J]. European Journal of Pharmaceutical Sciences, 2013(3-4):243-252.
[11] 罗宇燕, 麦海燕, 黎呐, 等. 复乳法及其改良法制备的干扰素PLGA微球载药释药特性的对比[J]. 中山大学学报(自然科学版), 2014, 53(3):110-114.
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